Effect of Osmolarity and Density of Colloid Formulations on the Outcome of SLC-Selection of Stallion Spermatozoa

Table 2

Effect of osmolarity of colloid on normal sperm morphology and %DNA fragmentation index (mean ± SD) (𝑛=12).

Parameter	Control	SLC normal	SLC high	DGC normal	DGC high
Normal morphology (%)	68.2±14.8^a	78±7.9^ab	82.8±9.0^ab	78±8.2^ab	79.4±8.1^a
%DFI	12.4±4.4^a	6.4±5.4^a	5.9±4.2^a	5.3±3.0^a	5.0±4.3^a

aControl significantly different to other treatments 𝑃<.001; bSLC high > SLC normal and SLC high > DGC normal (𝑃<.05 for both). SLC: single layer centrifugation, DGC: gradient density centrifugation.