International Scholarly Research Notices / 2011 / Article / Tab 1

Research Article

Single Layer Centrifugation Can Be Scaled-Up Further to Process up to 150 mL Semen

Table 1

Summary of the volumes of colloid and extended semen*, centrifuge tubes, and centrifuges used in these experiments (modified from [14]).

SpeciesTube sizeVolume of colloid (mL)Volume of extended semen* (mL)Name of techniqueCentrifuge

Stallion/boar12 mL4.04.5SLC-4.51
Stallion/boar50 mL1515–18SLC-151
Stallion/boar100 mL2025SLC-252
Stallion/boar200 mL6060SLC-603
Boar**500 mL150150SLC-1501

*Extended to give a concentration of approximately 100 × 106 spermatozoa/mL.
**this technique has not been attempted yet with stallion semen but is theoretically possible.
Centrifuge 1: for example: Hereaus Multifuge, Kendro, Osterode, Germany;
Centrifuge 2: for example: Sigma Laboratory Centrifuge, Osterode, Germany;
Centrifuge 3: for example: Centra MP 4R, International Equipment Company, MA, USA.

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