|*Extended to give a concentration of approximately 100 × 106 spermatozoa/mL.|
**this technique has not been attempted yet with stallion semen but is theoretically possible.
Centrifuge 1: for example: Hereaus Multifuge, Kendro, Osterode, Germany;
Centrifuge 2: for example: Sigma Laboratory Centrifuge, Osterode, Germany;
Centrifuge 3: for example: Centra MP 4R, International Equipment Company, MA, USA.