Assessment of Styrene Oxide Neurotoxicity Using In Vitro Auditory Cortex Networks

Figure 5

Subchronic effects of 2.0 mM SO (in 2% DMSO) on ACN spontaneous activity and morphology. (a) Mean spike rate per min from 24 units show initial cessation of activity and slow recovery of activity lasting for about an hour that was abolished in 3.5 hrs. A complete wash did not lead to any recovery of activity. (b) and (c) Neurons and glia in reference medium (1) and after 5 hrs in 2.0 mM SO (2). Although some neuronal death is present (arrows) the most obvious response is a massive glial swelling. (b) and (c) Are different networks. c2 is not the same area as c1. Arrows in (b) point to reference neurons for orientation.