Evaluation of the In Situ Hybridization Signal Patterns of Liquid-Based Cytological Human Papillomavirus Specimens for Diagnosing Squamous Intraepithelial Lesion
Table 1
A list of the case studied.
Histology
Case no.
Age
PCR (HPV genotype)
Cytology
HE
h-ISH
Papacicolaou
c-ISH
Superficial
Intermediate
Basal
1
40
−
NILM
−
metaplasia
−
−
−
2
31
53
NILM
−
metaplasia
−
−
−
3
22
31
NILM
−
sq. epithelium
−
−
−
4
35
58
LSIL
+
CIN1
I/E(+)
I(÷)
I(÷)
5
27
18/58
LSIL
+
CIN1
E/I(+)
E/I(+)
I(÷)
6
45
6/18
LSIL
+
CIN1
−
−
−
7
25
56
LSIL
+
CIN1
E/I(+)
E/I(+)
I(÷)
8
26
51
LSIL
+
CIN1
−
−
−
9
28
16
LSIL
+
*CIN3 (CIS)
I(+)
I(+)
I(+)
10
30
52
LSIL
−
CIN3
I(÷)
I(÷)
I(÷)
11
29
18
LSIL
+
CIN1
E/I(+)
E/I(+)
I(+)
12
24
66
LSIL
+
CIN1
E/I(+)
E/I(+)
−
13
30
16
LSIL
+
CIN1
−
−
I(÷)
14
52
53
LSIL
+
CIN1
−
−
−
15
39
52
LSIL
+
CIN2
−
−
−
16
26
−
LSIL
−
cervicitis
−
−
−
17
38
16
ASC-US
+
CIN1
E(+)
E(+)
I(÷)
18
38
52
ASC-US
−
CIN1
E(+)
E(+)
−
19
57
−
ASC-US
−
metaplasia
−
−
−
20
48
−
ASC-US
−
cervicitis
−
−
−
21
38
−
ASC-US
−
cervicitis
−
−
−
22
47
59
HSIL (moderate)
+
CIN1
−
−
−
23
29
16
HSIL (moderate)
−
CIN1
−
−
−
24
42
−
HSIL (moderate)
+
CIN3
I(+)
I(+)
I(+)
25
60
16/18/68
HSIL (moderate)
+
CIN3
I(÷)
I(÷)
I(÷)
26
24
66
HSIL (moderate)
+
*CIN3
I(÷)
I(÷)
I(÷)
27
30
16/33
HSIL (moderate)
+
CIN3
I/E(+)
I(+)
I(+)
28
26
16
HSIL (severe)
+
CIN3
I(+)
I(+)
I(+)
29
64
52
HSIL (severe)
+
CIN3
I(÷)
I(÷)
I(÷)
30
29
16
HSIL (severe)
+
CIN3
E(+)
E/I(+)
I(+)
31
41
52
HSIL (CIS)
+
CIN3 (CIS)
I/E(+)
I(+)
I(+)
32
25
16
HSIL (CIS)
+
CIN3 (CIS)
I/E(+)
I(+)
I(+)
33
30
16
HSIL (CIS)
+
CIN3 (CIS)
E/I(+)
I/E(+)
I(+)
Abbreviations: NILM, negative for intraepithelial lesion; sq., squamous; LSIL, low grade intraepithelial neoplasia; ASC-US, atypical squamous cells of undetermined significance; HSIL, high grade intraepithelial neoplasia; CIN, cervical intraepithelial neoplasia; CIS, carcinoma in situ; PCR, polymerase chain reaction; c-ISH, cytological in situ hybridization; h-ISH, histological ISH; superficial, superficial layer; intermediate, intermediate layer; basal, basal laer in cervical epithelial layer; E, episomal pattern; I, integrated pattern. The intensity of expression of above items was evaluated on an arbitrary scale from − (no detectable staining), (weak staining intensity), to + (significant staining intensity). In hISH, E/I indicates the E-signal is more than I-signal, I/E indicates the I-signal more than E-signal.
*In the case 9 and 26 (CIN3 cases), CIN1 lesions are also observed in the same histological specimen.