Evaluation of the In Situ Hybridization Signal Patterns of Liquid-Based Cytological Human Papillomavirus Specimens for Diagnosing Squamous Intraepithelial Lesion

Table 1

A list of the case studied.

					Histology
Case no.	Age	PCR (HPV genotype)	Cytology		HE		h-ISH
			Papacicolaou	c-ISH		Superficial	Intermediate	Basal
1	40	−	NILM	−	metaplasia	−	−	−
2	31	53	NILM	−	metaplasia	−	−	−
3	22	31	NILM	−	sq. epithelium	−	−	−
4	35	58	LSIL	+	CIN1	I/E(+)	I(÷)	I(÷)
5	27	18/58	LSIL	+	CIN1	E/I(+)	E/I(+)	I(÷)
6	45	6/18	LSIL	+	CIN1	−	−	−
7	25	56	LSIL	+	CIN1	E/I(+)	E/I(+)	I(÷)
8	26	51	LSIL	+	CIN1	−	−	−
9	28	16	LSIL	+	*CIN3 (CIS)	I(+)	I(+)	I(+)
10	30	52	LSIL	−	CIN3	I(÷)	I(÷)	I(÷)
11	29	18	LSIL	+	CIN1	E/I(+)	E/I(+)	I(+)
12	24	66	LSIL	+	CIN1	E/I(+)	E/I(+)	−
13	30	16	LSIL	+	CIN1	−	−	I(÷)
14	52	53	LSIL	+	CIN1	−	−	−
15	39	52	LSIL	+	CIN2	−	−	−
16	26	−	LSIL	−	cervicitis	−	−	−
17	38	16	ASC-US	+	CIN1	E(+)	E(+)	I(÷)
18	38	52	ASC-US	−	CIN1	E(+)	E(+)	−
19	57	−	ASC-US	−	metaplasia	−	−	−
20	48	−	ASC-US	−	cervicitis	−	−	−
21	38	−	ASC-US	−	cervicitis	−	−	−
22	47	59	HSIL (moderate)	+	CIN1	−	−	−
23	29	16	HSIL (moderate)	−	CIN1	−	−	−
24	42	−	HSIL (moderate)	+	CIN3	I(+)	I(+)	I(+)
25	60	16/18/68	HSIL (moderate)	+	CIN3	I(÷)	I(÷)	I(÷)
26	24	66	HSIL (moderate)	+	*CIN3	I(÷)	I(÷)	I(÷)
27	30	16/33	HSIL (moderate)	+	CIN3	I/E(+)	I(+)	I(+)
28	26	16	HSIL (severe)	+	CIN3	I(+)	I(+)	I(+)
29	64	52	HSIL (severe)	+	CIN3	I(÷)	I(÷)	I(÷)
30	29	16	HSIL (severe)	+	CIN3	E(+)	E/I(+)	I(+)
31	41	52	HSIL (CIS)	+	CIN3 (CIS)	I/E(+)	I(+)	I(+)
32	25	16	HSIL (CIS)	+	CIN3 (CIS)	I/E(+)	I(+)	I(+)
33	30	16	HSIL (CIS)	+	CIN3 (CIS)	E/I(+)	I/E(+)	I(+)

Abbreviations: NILM, negative for intraepithelial lesion; sq., squamous; LSIL, low grade intraepithelial neoplasia; ASC-US, atypical squamous cells of undetermined significance;
HSIL, high grade intraepithelial neoplasia; CIN, cervical intraepithelial neoplasia; CIS, carcinoma in situ; PCR, polymerase chain reaction; c-ISH, cytological in situ hybridization; h-ISH, histological ISH; superficial, superficial layer; intermediate, intermediate layer; basal, basal laer in cervical epithelial layer; E, episomal pattern; I, integrated pattern.
The intensity of expression of above items was evaluated on an arbitrary scale from − (no detectable staining), ÷ (weak staining intensity), to + (significant staining intensity).
In hISH, E/I indicates the E-signal is more than I-signal, I/E indicates the I-signal more than E-signal.
*In the case  9 and 26 (CIN3 cases), CIN1 lesions are also observed in the same histological specimen.