Research Article

Investigation of Archived Formalin-Fixed Paraffin-Embedded Pancreatic Tissue with Whole-Genome Gene Expression Microarray

Figure 1

Scatter plot of the vsn-normalized and l o g 2 -transformed signal intensities. (a) Scatter plot of the l o g 2 -transformed signal intensities of insulinoma samples (Cy5) and reference material (Cy3). Correlation coefficient (CC) for each scatter plot (sample versus reference material) is shown on the top, confirming the very uniform spot distribution. (b) Scatter plot of the l o g 2 -transformed signal intensities: square root of sample multiplied reference ( 𝑦 -axes) against ratio of sample versus reference ( 𝑥 -axes). The data distribute around a horizontal line at 0. CC reprod_FFPE for the two FFPE samples was 0.86, whereas CCreprod_FF_FFPE for FF and FFPE tissues was 0.63.
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275102.fig.001b
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