Investigation of Archived Formalin-Fixed Paraffin-Embedded Pancreatic Tissue with Whole-Genome Gene Expression Microarray

Table 2

Total RNA after DNase treatment and labelling with fluorophore. RNA quality and labelling efficiency were calculated for FF and FFPE samples (nt nucleotides, aaRNA aminoallyl-modified RNA).

		RNA			N dye mol incorp/1000 nt	pmol dye incorp/ng labelled aaRNA
		Purity	Conc. μg/μL	Quant μg
All	Mean	1.83	0.19	22.36	42.9	0.119
	Min.		0.08	7.20	35.30	0.098
	Max.		0.59	76.65	66.80	0.185
FFPE	Mean	1.84	0.21	24.96	43.7	0.121
	Min.		0.10	9.04	35.30	0.098
	Max.		0.59	76.65	66.80	0.185
Frozen	Mean	1.77	0.13	11.95	39.7	0.110
	Min.		0.08	7.20	37.70	0.105
	Max.		0.16	14.33	42.50	0.118