Culture Models for Studying Thyroid Biology and Disorders
Figure 7
Models for analyzing thyrocyte-other cell type interaction. (a) Tg solution is overlaid on the surface of thyrocytes monolayer-cultured on other cell type-embedded collagen gel layer in an inner dish (1) with nitrocellulose membrane in its bottom. The inner dish is placed in a larger outer dish (2), and then culture medium is added to the outer dish. (b) To easily analyze the protein, mRNA, and DNA expression of both thyrocytes and other cell types under their interactions, the inner dish, in which Tg solution-exposing thyrocytes are cultured on acellular collagen gel layer, is placed in the outer dish where other cell types are cultured in a monolayer or 3-D collagen gel.
