Table of Contents
ISRN Microbiology
Volume 2011, Article ID 318348, 5 pages
http://dx.doi.org/10.5402/2011/318348
Research Article

Prevalence of ESBL-Producing Klebsiella pneumoniae Isolates in Tertiary Care Hospital

1Department of Microbiology, Government Medical College, Anantapur 515001, India
2Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa 516003, India

Received 25 August 2011; Accepted 19 October 2011

Academic Editors: A. Netrusov and P. Zunino

Copyright © 2011 Vemula Sarojamma and Vadde Ramakrishna. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Extended-spectrum β lactamases (ESBLs) continue to be a major challenge in clinical setups world over, conferring resistance to the expanded-spectrum cephalosporins. An attempt was made to study the prevalence of ESBL-producing Klebsiella pneumoniae clinical isolates in a tertiary care hospital in Kurnool. A total of hundred collected isolates of Klebsiella pneumoniae was studied for their susceptibility patterns to various antibiotics and detection of ESBL producers by double disc synergy test (DDST) and phenotypic confirmatory disc diffusion test (PCDDT). Of the 100 isolates tested for their antibiogram, 61% isolates have shown susceptibility to 3rd-generation cepholosporins and 39% were resistant. Amoxycillin showed the highest percentage of resistance followed by tetracyclins and cotrimoxazole. Among 39 resistant isolates of Klebsiella pneumoniae, 17 were ESBL producers detected by DDST and PCDDT. ESBL producers were more in the hospital isolates (28%) compared to community isolates (6%). Maximum percentage of ESBL producers were noticed from blood sample with 57.14%. In the present study, a large number of isolates were found to be multidrug resistant and ESBL producers. PCDDT was found to be better than DDST in the detection of ESBLs. Continued monitoring of drug resistance is necessary in clinical settings for proper disease management.