Table of Contents
ISRN Renewable Energy
Volume 2011 (2011), Article ID 328984, 6 pages
http://dx.doi.org/10.5402/2011/328984
Research Article

Influence of Different Cultural Conditions on Photoproduction of Hydrogen by Rhodopseudomonas palustris KU003

1University College of Science and Informatics, Mahatma Gandhi University, Nalgonda 508001, India
2Department of Biochemistry, Osmania Unviersity, Hyderabad 500007, India
3Department of Biochemistry and Microbiology, Kakatiya University, Warangal 506009, India

Received 8 October 2011; Accepted 23 November 2011

Academic Editor: A. Bosio

Copyright © 2011 Ramchander Merugu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Photoproduction of hydrogen by Rhodopseudomonas palustris KU003 under different cultural conditions with various carbon and nitrogen sources was investigated. Hydrogen production was measured using a Gas chromatograph. Malate promoted more amounts of hydrogen production under anaerobic light conditions than anaerobic dark conditions. Cumulative hydrogen production by the organism was recorded at various time intervals. Incubation period of 120 hrs was optimum for production of hydrogen. pH 7 . 0 ± 0 . 4 was optimum for production of hydrogen. L-glutamic acid was a good nitrogen source for production of hydrogen. Growing cells produced more amount of hydrogen than resting cells. Significance of the above results in presence of existing literature is discussed.