Research Article

Influence of Different Cultural Conditions on Photoproduction of Hydrogen by Rhodopseudomonas palustris KU003

Table 3

Effect of nitrogen sources on hydrogen production (mL/15 mL vessel) by R. palustris under anaerobic light.

Nitrogen sourceIncubation period (in hrs)
24487296120144166192

Potassium nitrateR 0 . 2 2 ± 0 . 0 4 0 . 4 2 ± 0 . 0 8 0 . 6 6 ± 0 . 1 0 0 . 3 6 ± 0 . 0 8
G 0 . 4 6 ± 0 . 0 8 0 . 8 8 ± 0 . 0 4 1 . 2 2 ± 0 . 1 0 0 . 6 2 ± 0 . 1 8 0 . 3 2 ± 0 . 0 4
Sodium nitrateR 0 . 3 6 ± 0 . 0 6 0 . 5 2 ± 0 . 0 8 0 . 7 6 ± 0 . 1 0 0 . 4 8 ± 0 . 1 2
G 0 . 4 2 ± 0 . 0 8 0 . 5 4 ± 0 . 1 4 0 . 8 4 ± 0 . 1 2 1 . 2 6 ± 0 . 1 4 1 . 8 6 ± 0 . 1 8 1 . 4 4 ± 0 . 2 8 1 . 0 8 ± 0 . 1 6 0 . 6 6 ± 0 . 0 6
Ammonium chlorideR
G
UreaR 0 . 3 4 ± 0 . 0 4
G 0 . 3 8 ± 0 . 0 8 0 . 6 6 ± 0 . 0 8 0 . 8 8 ± 0 . 1 2 0 . 5 2 ± 0 . 1 6 0 . 3 8 ± 0 . 0 4
ThioureaR
G 0 . 3 8 ± 0 . 1 8 0 . 5 4 ± 0 . 2 8 0 . 3 4 ± 0 . 0 2 0 . 2 2 ± 0 . 0 6
GlycineR 0 . 2 8 ± 0 . 0 8 0 . 4 8 ± 0 . 1 0 0 . 3 6 ± 0 . 0 6
G 0 . 5 4 ± 0 . 2 0 0 . 9 6 ± 0 . 1 2 1 . 3 ± 0 . 1 8 0 . 7 4 ± 0 . 1 4 0 . 4 6 ± 0 . 1 0
L-asparagineR 0 . 2 4 ± 0 . 1 6 0 . 4 4 ± 0 . 1 2 0 . 6 6 ± 0 . 0 8 0 . 3 6 ± 0 . 0 6
G 0 . 4 6 ± 0 . 0 2 0 . 8 4 ± 0 . 1 2 1 . 2 6 ± 0 . 0 6 1 . 7 4 ± 0 . 1 8 1 . 4 6 ± 0 . 1 2 1 . 1 6 ± 0 . 0 6 0 . 6 8 ± 0 . 0 6
L-aspartic acidR 0 . 3 6 ± 0 . 1 8 0 . 6 8 ± 0 . 1 8 0 . 9 6 ± 0 . 0 8 0 . 5 8 ± 0 . 2 0 0 . 2 6 ± 0 . 0 8
G 0 . 5 2 ± 0 . 0 6 0 . 7 6 ± 0 . 0 8 1 . 3 4 ± 0 . 0 8 1 . 8 4 ± 0 . 0 8 2 . 2 ± 0 . 0 8 1 . 5 4 ± 0 . 0 8 1 . 0 8 ± 0 . 1 8 0 . 6 6 ± 0 . 1 2
L-glutamic acidR 0 . 6 6 ± 0 . 0 4 0 . 8 8 ± 0 . 0 8 1 . 2 2 ± 0 . 1 0 0 . 9 8 ± 0 . 1 2 0 . 7 2 ± 0 . 0 4
G 0 . 4 8 ± 0 . 1 0 0 . 9 6 ± 0 . 1 6 1 . 5 4 ± 0 . 1 2 1 . 8 6 ± 0 . 0 8 2 . 5 6 ± 0 . 0 6 2 . 2 2 ± 0 . 0 8 1 . 7 6 ± 0 . 0 8 0 . 8 4 ± 0 . 0 8
L-glutamineR 0 . 4 8 ± 0 . 1 2 0 . 7 8 ± 0 . 0 4 0 . 5 6 ± 0 . 0 6 0 . 3 4 ± 0 . 1 0
G 0 . 4 2 ± 0 . 0 8 0 . 7 6 ± 0 . 1 4 1 . 1 6 ± 0 . 2 4 1 . 5 8 ± 0 . 1 6 1 . 2 4 ± 0 . 2 8 0 . 8 8 ± 0 . 1 4 0 . 5 4 ± 0 . 0 2
L-tyrosineR 0 . 3 2 ± 0 . 0 2 0 . 4 6 ± 0 . 2 2 0 . 8 4 ± 0 . 2 8 0 . 5 8 ± 0 . 0 6 0 . 2 8 ± 0 . 1 4
G 0 . 3 8 ± 0 . 0 4 0 . 7 8 ± 0 . 0 6 1 . 1 8 ± 0 . 1 4 1 . 5 6 ± 0 . 1 4 2 . 3 0 ± 0 . 0 8 1 . 7 8 ± 0 . 1 2 1 . 3 4 ± 0 . 1 6 0 . 5 8 ± 0 . 0 4
L-cystineR 0 . 3 2 ± 0 . 0 4 0 . 5 4 ± 0 . 0 8 1 . 1 0 ± 0 . 0 8 0 . 6 8 ± 0 . 1 2 0 . 3 8 ± 0 . 1 4
G 0 . 3 0 ± 0 . 0 4 0 . 7 6 ± 0 . 1 0 1 . 3 4 ± 0 . 1 2 1 . 6 4 ± 0 . 1 4 1 . 9 4 ± 0 . 2 8 1 . 4 8 ± 0 . 2 4 1 . 1 0 ± 0 . 2 2 0 . 5 4 ± 0 . 1 6
L-methionineR 0 . 3 4 ± 0 . 0 6 0 . 6 4 ± 0 . 0 4 0 . 4 0 ± 0 . 0 8
G 0 . 4 6 ± 0 . 0 4 0 . 8 4 ± 0 . 3 2 1 . 2 4 ± 0 . 2 6 0 . 9 6 ± 0 . 1 0 0 . 5 6 ± 0 . 1 2
Nitrogen gasR 0 . 2 8 ± 0 . 0 2
G 0 . 2 8 ± 0 . 1 0 0 . 6 6 ± 0 . 1 8 1 . 1 2 ± 0 . 3 2 0 . 4 2 ± 0 . 1 6

R: resting cells; G: growing cells; —: no hydrogen production.