Table of Contents
ISRN Obstetrics and Gynecology
Volume 2011, Article ID 354861, 7 pages
Research Article

Whole Slide Quantification of Stromal Lymphatic Vessel Distribution and Peritumoral Lymphatic Vessel Density in Early Invasive Cervical Cancer: A Method Description

1Laboratory of Tumor and Development Biology, Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA-Cancer), University of Liège, Pathology Tower (B23), 4000 Liège, Belgium
2Department of Obstetrics and Gynecology, Hospital of la Citadelle, 4000 Liège, Belgium
3Department of Obstetrics and Gynecology, CHU of Liège, 4000 Liège, Belgium

Received 14 April 2011; Accepted 20 June 2011

Academic Editor: Y. S. Song

Copyright © 2011 C. Balsat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Peritumoral Lymphatic Vessel Density (LVD) is considered to be a predictive marker for the presence of lymph node metastases in cervical cancer. However, when LVD quantification relies on conventional optical microscopy and the hot spot technique, interobserver variability is significant and yields inconsistent conclusions. In this work, we describe an original method that applies computed image analysis to whole slide scanned tissue sections following immunohistochemical lymphatic vessel staining. This procedure allows to determine an objective LVD quantification as well as the lymphatic vessel distribution and its heterogeneity within the stroma surrounding the invasive tumor bundles. The proposed technique can be useful to better characterize lymphatic vessel interactions with tumor cells and could potentially impact on prognosis and therapeutic decisions.