Table of Contents
ISRN Microbiology
Volume 2011, Article ID 458294, 4 pages
http://dx.doi.org/10.5402/2011/458294
Research Article

Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis

1LANAGRO/MG, Avenida Rômulo Joviano, Caixa Postal 50, Centro, 33600-000 Pedro Leopoldo, Minas Gerais, CEP, Brazil
2CEDISA, Concórdia, Santa Catarina, Brazil
3Embrapa Suínos e Aves, Concórdia, Santa Catarina, Brazil
4Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

Received 18 September 2011; Accepted 9 October 2011

Academic Editor: T. Krishnan

Copyright © 2011 Antônio Augusto Fonseca Jr. et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky's disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1.