Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12

Table 1

Weighted mean COV and NormFinder stability index of 39 invariant genes across 3 datasets (MG1655).

Gene symbol	Gene name	Weighted COV values	Weighted NormFinder Stability Index
mdoG	Glucan biosynthesis protein G	0.099	0.082
dapA	Dihydrodipicolinate synthase	0.106	0.090
crp	DNA-binding transcriptional dual regulator	0.106	0.102
hslV	Peptidase component of the HslUV protease	0.111	0.105
mrdB	Cell wall shape-determining protein	0.101	0.114
fucU	L-Fucose mutarotase	0.107	0.117
yjgP	LPS transport (lptF)	0.105	0.117
yigC	3-Octaprenyl-4-hydroxybenzoate decarboxylase	0.126	0.117
sun	16S rRNA m(5)C967 methyltransferase, S-adenosyl-L-methionine-dependent	0.130	0.119
gor	Glutathione oxidoreductase	0.117	0.126
hflB	ATP-dependent metalloprotease	0.127	0.130
yqiB	Predicted dehydrogenase	0.125	0.134
murG	N-Acetylglucosaminyl transferase	0.124	0.134
yrbG	Predicted calcium/sodium:proton antiporter	0.122	0.134
yejK	Nucleotide associated protein	0.120	0.141
yfgA	Cytoskeletal protein required for MreB assembly	0.118	0.142
hflX	Putative GTPase HflX	0.105	0.142
spoT	Bifunctional (p)ppGpp synthetase II/guanosine-; 3′,5′-bis pyrophosphate 3′-pyrophosphohydrolase	0.117	0.143
holC	DNA polymerase III, chi subunit	0.134	0.144
xerD	Site-specific tyrosine recombinase	0.114	0.146
tolB	Periplasmic protein	0.115	0.146
yheS	Fused predicted transporter subunits of ABC superfamily: ATP-binding components	0.110	0.146
ntpA	Dihydroneopterin triphosphate pyrophosphatase	0.118	0.147
yabB	Conserved protein, MraZ family	0.115	0.148
lolA	Chaperone for lipoproteins	0.117	0.153
yggD	Predicted DNA-binding transcriptional regulator	0.116	0.153
pnp	Polynucleotide phosphorylase/polyadenylase	0.110	0.155
yrbB	ABC transporter maintaining OM lipid asymmetry, cytoplasmic STAS component	0.123	0.156
rnc	RNase III	0.117	0.157
xerC	Site-specific tyrosine recombinase	0.138	0.160
rfaF	ADP-heptose:LPS heptosyltransferase II	0.120	0.161
yigP	Conserved protein, SCP2 family	0.122	0.164
gyrB	DNA gyrase, subunit B	0.126	0.164
nagC	DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine	0.132	0.165
nrdR	Conserved protein	0.118	0.168
hemD	Uroporphyrinogen III synthase	0.108	0.169
pheT	Phenylalanine tRNA synthetase, beta subunit	0.124	0.171
frr	Ribosome recycling factor	0.129	0.173
cls	Cardiolipin synthase 1	0.129	0.181