Table of Contents
ISRN Veterinary Science
Volume 2011, Article ID 501235, 4 pages
Research Article

Detection of Paratuberculosis in Breeding Bulls at Pakistani Semen Production Units: A Continuous Source of Threat

1Quality Control Laboratory, Veterinary Research Institute, Lahore, Pakistan
2Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden
3Department of Natural Sciences, University of Skövde, 541 28 Skövde, Sweden

Received 14 November 2011; Accepted 7 December 2011

Academic Editors: R. Harasawa and R. Thanawongnuwech

Copyright © 2011 Muhammad Abbas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Paratuberculosis is a chronic bowel disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its secretion through semen highlights the importance of paratuberculosis-free breeding bulls. The breeding and teaser bulls at three semen production units (SPUs) located in Punjab, Pakistan, were screened for the presence of antibodies against MAP. A total of 253 samples were collected from SPUs and a commercially available indirect screen ELISA (Is-ELISA) was applied. Is-ELISA detected antibodies in 20 (24.6%), 16 (22.8%), and 17 (16.6%) samples from SPU-I, SPU-II, and SPU-III, respectively. Collectively, seroprevalence of 20.0% (47/235) in breeding bulls and 33.3% (6/18) in teaser bulls was observed, and thus it poses a potential threat of disease spread to a high number of heifers and cows through artificial insemination. Therefore, this paper highlights the presence of the disease for the first time at SPUs and triggers attempts to ascertain the prevalence of paratuberculosis throughout the country.