Familial Parkinson's Disease Mutant E46K α-Synuclein Localizes to Membranous Structures, Forms Aggregates, and Induces Toxicity in Yeast Models
Figure 1
E46K exhibits species- and strain-specific toxicity in yeasts. (a) LEFT—BY4741 (mat a) and BY4743 (diploid mat a/α) budding yeast cells expressing WT, E46K, A30P, or A53T α-synuclein were serially diluted 5-fold and spotted onto repressing (glucose) or inducing (galactose) plates and grown for two days. Vector alone and GFP served as controls. No E46K-dependent α-synuclein toxicity was observed in either strain. (b) LEFT—TCP1 and SP3 fission yeast cells expressing WT, E46K, A30P, or A53T α-synuclein were serially diluted 5-fold and spotted onto repressing (+thiamine) or inducing (−thiamine) plates. Similar to budding yeast, vector alone and GFP served as controls. In both strains, WT, E46K, and A53T were toxic, but not A30P. In SP3, E46K was more toxic than WT.
