Familial Parkinson's Disease Mutant E46K α-Synuclein Localizes to Membranous Structures, Forms Aggregates, and Induces Toxicity in Yeast Models
Figure 5
E46K α-synuclein expression in budding and fission yeasts. (a) Western blot of α-synuclein in budding yeast lysates of BY4741 (left) and BY4743 (right) at 24 and 48 hours of expression. E46K expression is similar to WT in BY4741 or lesser in BY4743. (b) Western blot of α-synuclein in fission yeast lysates of TCP1 (left) and SP3 (right) at 24 and 48 hours of expression. E46K expression is more than WT in TCP1 (24 hrs) and SP3 (24 and 48 hrs). (c) Western blot of budding yeast (BY4741) and fission yeast (TCP1 and SP3) expressing WT and E46K for 24 hours, probed with serine-129 phosphorylation-specific antibody (ab59264; Abcam), V5 antibody (for total α-synuclein), and β-actin (protein loading control). WT and E46K are phosphorylated at serine-129 in both yeasts.
