Table of Contents
ISRN Dentistry
Volume 2011 (2011), Article ID 543561, 6 pages
http://dx.doi.org/10.5402/2011/543561
Clinical Study

Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood

1Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA
2Department of Pediatric Dentistry, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA
3Center for Craniofacial and Dental Genetics, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA
4Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA
5Clinical and Translational Science Institute, University of Pittsburgh, Pittsburgh, PA, USA
6Dental Public Health/Information Management, University of Pittsburgh, Pittsburgh, PA, USA
7Department of Pedodontics, Istanbul Medipol University, Istanbul, Turkey
8Department of Pedodontics, Istanbul University, Istanbul, Turkey
9ECLAMC, Center for Medical Education and Clinical Research(CEMIC), Buenos Aires, Argentina
10ECLAMC, National Institute of Population Medical Genetics(INAGEMP-CNPq) CEMIC, Buenos Aires, Argentina
11ECLAMC, Hospital de Area El Bolsón, Río Negro, Argentina
12ECLAMC, I Multidisciplinary Institute of Cellular Biology (MBICE), La Plata, Argentina
13ECLAMC, National Institute of Population Medical Genetics (INAGEMP-CNPq) Department of Genetics, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil
14Department of Genetics, Institute of Biology, Center of Health Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
15Department of Periodontics, School of Dentistry, West Virginia University, Morgantown, WV, USA
16Department of Dental Practice and Rural Health, West Virginia University, Morgantown, WV, USA
17Department of Psychiatry, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA

Received 5 January 2011; Accepted 1 March 2011

Academic Editors: P. Gjermo and A. Jäger

Copyright © 2011 Alexandre R. Vieira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.