Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar Semen

Table 4

Proportion (%) (mean ± SD) of living, ROS producing, dead, and dying boar spermatozoa measured 24 h after semen collection and SLC (𝑛=16 ejaculates).

Parameter	Classification	Unselected (%)	SLC-4.5 (%)	SLC-15 (%)
	Living	94±2.7	92.8±3.2	94.4±2.5
SYBR14/PI	Dying	4.7±2.3	5.6±2.4	4.2±1.8
	Dead	1.3±0.6	1.4±0.7	1.2±0.8
	Living ROS −ve	85.1±3.6^ac	67.9±13.1^a	72.1±16.6^c
ROS	Living ROS +ve	4.8±1.7^bc	8.4±3.5^b	8.6±5.7^c
	Dead	9.6±2.6^a	23.1±9.5^a	18.9±10.9^b

Note: ROS = reactive oxygen species; SYBR14/PI staining for membrane integrity. ^a𝑃<.001; ^b𝑃<.01; ^c𝑃<.05.
For viability (SYBR14/PI), there were no statistically significant differences among treatments.