Table of Contents
ISRN Pharmacology
Volume 2011 (2011), Article ID 619395, 11 pages
http://dx.doi.org/10.5402/2011/619395
Research Article

Antioxidant Activity of Aspergillus fumigatus

Microbial Technology Laboratory, Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India

Received 14 January 2011; Accepted 21 February 2011

Academic Editors: H. Cerecetto and M. L. Ferrandiz

Copyright © 2011 Daljit Singh Arora and Priyanka Chandra. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The antioxidant activity of Aspergillus fumigatus was assayed by different procedures and correlated with its extracellular total phenolic contents. Different physio-chemical parameters were optimized to enhance the activity. The culture grown under stationary conditions for 10 days at 25°C at pH 7 gave the best antioxidant activity. Statistical approaches demonstrated sucrose and NaNO3 to be the most suitable carbon and nitrogen sources, respectively. Response surface analysis showed 5% sucrose, 0.05% NaNO3, and incubation temperature of 35°C to be the optimal conditions for best expression of antioxidant activity. Under these conditions, the antioxidant potential assayed through different procedures was 89.8%, 70.1%, and 70.2% scavenging effect for DPPH radical, ferrous ion and nitric oxide ion, respectively. The reducing power showed an absorbance of 1.0 and FRAP assay revealed the activity of 60.5%. Extracellular total phenolic content and antioxidant activity as assayed by different procedures positively correlated.