Table of Contents
ISRN Microbiology
Volume 2011 (2011), Article ID 697020, 5 pages
http://dx.doi.org/10.5402/2011/697020
Research Article

Reduction of Adherence of E. coli O157:H7 to HEp-2 Cells and to Bovine Large Intestinal Mucosal Explants by Colicinogenic E. coli

1Laboratorio de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro Provincia de Buenos Aires, Pinto 399 (7000), Tandil, Argentina
2Laboratorio de Histología, Departamento de Ciencias Biológicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro Provincia de Buenos Aires, Pinto 399 (7000), Tandil, Argentina

Received 9 August 2011; Accepted 20 September 2011

Academic Editors: H.-P. Horz and J. D. Stopforth

Copyright © 2011 A. I. Etcheverría et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Enterohemorrhagic E. coli strains (EHEC) had emerged as foodborne pathogens and cause in human diarrhea and hemolytic-uremic syndrome. Because of the widespread distribution of EHEC serotypes and O157 and non-O157 in cattle population, its control will require interventions at the farm level such as the administration of probiotics that produce inhibitory metabolites. E. coli O157:H7 shows tissue tropisms for the gastrointestinal tract (GIT) of cattle. The aim of this study was to test the ability of a colicinogenic E. coli (isolated from bovine) to reduce the adherence of E. coli O157:H7 to HEp-2 cells and to GIT of cattle. We inoculated HEp-2 cells and bovine colon explants with both kinds of strains. Colicinogenic E. coli was able to reduce the adherence of E. coli O157:H7 to HEp-2 cells and to bovine tissues.