Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies
Figure 1
Stable expression of the wild and mutant α-synuclein in vitro. Immunoblots of α-synuclein and β-actin in SH-SY5Y cells without transfection (Lane 1), transfected with empty vector (Lane 2), or vectors encoding the wild-type (Lane 3) or mutant (Lane 4) α-synuclein. Cell lysates were analyzed by immunoblotting using anti-α-synuclein antibody. β-actin was used as a loading control. The wild-type α-synuclein and the S129A α-synuclein protein in transfection groups were obviously higher than that in nontransfected and empty plasmid transfected groups.
