Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies
Figure 3
Decreased crosslinking between tTG and the mutant α-synuclein S129A. Cells were transfected with empty vectors, vectors encoding the wild-type or the mutant α-synuclein S129A. They were treated with 20 μM retinoic acid for 7 days and incubated for 24 h in the presence of 20 nM okadaic acid to generate tTG in the system. Immunoprecipitation was performed to access the interaction between tTG and α-synuclein. The mutant S129A showed significant reduction in the crosslinking between the tTG and the α-synuclein monomer and dimer. * compared with cells transfected with empty vectors.
