Table of Contents
ISRN Microbiology
Volume 2011, Article ID 797463, 4 pages
http://dx.doi.org/10.5402/2011/797463
Research Article

Genetic Diversity and Drug Resistance Mutations in HIV-1 from Untreated Patients in Niamey, Niger

1Laboratory of Bacteriology-Virology, Faculty of Health Sciences, Abdou Moumouni University, P.O. Box 237, Niamey, Niger
2National Reference Laboratory for STI/HIV/TB, P.O. Box 10 146, Niamey, Niger
3Centre de Traitement Ambulatoire (CTA) of Niamey, P.O. Box 11 236, Niamey, Niger
4Solidarité Thérapeutique et Initiative Contre le Sida (SOLTHIS), P.O. Box 10 393, Niamey, Niger

Received 10 August 2011; Accepted 17 September 2011

Academic Editors: A. L. Chenine and M. Shehu-Xhilaga

Copyright © 2011 Saïdou Mamadou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of the study was to estimate the prevalence of transmitted resistance to antiretroviral of HIV-1 circulating in Niger. We collected plasmas from 96 drug-naive patients followed up in the main HIV/AIDS Care Center of Niamey, the capital city of Niger. After RNA extraction and retrotranscription to proviral DNA, nested PCR was performed to amplify PR (codons 1–99) and RT (codons 1–240) fragments for sequencing. Sequences were analysed for phylogeny, then for resistance-associated mutations according to IAS-USA and Stanford's lists of mutations. We characterized six HIV-1 genetic variants: CRF02-AG (56.3%), CRF30_0206 (15.6%), subtype G (15.6%), CRF06_cpx (9.4%), CRF11_cpx (2.1%), and CRF01_AE (1%). About 8.3% of HIV strains had at least 1 resistance mutation: 4 strains with at least 1 mutation to NRTI, 5 for NNRTI, and 1 for PI, respectiveley 4.2%, 5.2%, and 1.0%. These preliminary results gave enough information for the need of instauring HIV drug resistance national surveillance.