Irrigation Water Chemistry: Impact on Microbial Community Composition and Biogeochemical Leaching under Perennial Ryegrass (Lolium perenne [L])

Table 2

Univariate analysis of variance on individual FAME biomarkers to examine the effect of irrigation source water, starter fertilizer and interactions between irrigation water and fertilizer. Bold values show a statistically significant effect at 𝛼<0.05. + indicates an increase; − indicates a decrease and = indicates no significant increase or decrease. GWB is grey water-bath, GWW is grey water-washing machine, MTW is Municipal tap water, and ALL indicates all irrigation waters except harvested rain water.

	Fame	Water source	Fertilizer	Water*fertilizer interaction	Relative abundanc range (%)	Significant increase or decrease*
	ISO 15 : 0	0.68	0.97	0.12	4.6–4.9	=
Gram + bacteria	ANTEISO 15 : 0	0.05	0.86	0.2	2.0–2.3	+GWB
	15 : 0	0.37	0.53	0.75	0–0.2	=
	ISO 16 : 0	0.56	0.19	0.19	2.0–2.1	=
	16 : 0	0.00	0.76	0.42	17–20	−ALL
	ISO 17 : 1G	0.01	0.78	0.03	2.9–3.5	+GWW
	ISO 17 : 0	0.06	0.61	0.05	2.0–2.3	=
	ANTEISO 17 : 0	0.25	0.55	0.96	0.8–1.6	=
Gram− bacteria	16:1 ω9c	0.41	0.42	0.23	0–0.3	=
	16 : 1 ω7c	0.00	0.58	0.04	3.5–4.6	−ALL
	16 : 1 ω5c	0.00	0.04	0.01	4.9–8.3	=
	17 : 0 CYCLO	0.99	0.53	0.07	0.36–0.45	=
	18 : 1 ω5c	0.45	0.64	0.05	0–0.9	=
	19 : 0 CYCLO c11-12	0.00	0.32	0.06	3.8–5.7	+ALL
Fungi	18 : 2 ω6c	0.00	0.63	0.14	6.3–8.0	−ALL
	18 : 1 ω9c	0.01	0.67	0.87	14.5–17.7	−MTW/GWB
Protozoa	20 : 4 ω6c	0.13	0.81	0.95	0–0.4	=
Bacteria	17 : 0	0.15	0.06	0.25	0.4–1.2	=
	18 : 0	0.03	0.16	0.11	11.0–13.5	+GWB
	14 : 0	0.36	0.48	0.65	0.5–1.1	=
Other	Sum in feature 8	0.07	0.33	0.11	9.3–10.6	=
	19 : 1 Alcohol	0.67	0.96	0.50	0–0.3	=
	19 : 2 ω6c	0.94	0.67	0.10	0.5–0.9	=
	cis 9,10 epoxy 18 : 0	0.81	0.56	0.21	0.6–1.3	=
	20 : 1 ω9c	0.47	0.96	0.05	0.1–0.6	=
	20 : 0	0.05	0.39	0.3	0.7–1.7	=

*Significant increase or decrease is relative to soil microbes in harvested rain water-treatments at (𝛼<0.05).