Figure 2: Leukocyte recruitment to the bronchoalveolar space and determination of serum ovalbumin-specific IgE. BALB/c mice were sensitized with ovalbumin (OVA) in Al (OH)3 (day 0) and with OVA on day 14. They were subjected to drink water (control group and aerosol group) or OVA (oral group and oral/aerosol group) for 20 days (days 21 to 41). For 6 days (days 36 to 41), mice (aerosol group and oral/aerosol group) were aerosolized with OVA or saline (control group and oral group). (a) Total cells present in the BAL (b) airway eosinophilia, (c) activity of EPO in lungs, and (d) serum ovalbumin-specific IgE. Values represent the means +/− SEM, 𝑛 = 5 , representative of 2 experiments; *significant difference ( 𝑃 < 0 . 0 5 ) aerosol group versus control group, ** aerosol group versus oral/aerosol group.