Figure 3: Lung IL-4, CCL-2, CCL-11, IL-10 levels, and Foxp3 mRNA expression. BALB/c mice were sensitized with ovalbumin (OVA) in Al(OH)3 (day 0) and with OVA on day 14. They were subjected to drink water (control group and aerosol group) or OVA (oral group and oral/aerosol group) for 20 days (days 21 to 41). For 6 days (days 36 to 41), mice (aerosol group and oral/aerosol group) were aerosolized with OVA or saline (control group and oral group). (a) Levels of IL-4. (b) Levels of CCL-2. (c) Levels of CCL-11. (d) Levels of IL-10. (e) Increased expression of Foxp3 mRNA. Values represent the means +/− SEM, 𝑛 = 5 , representative of 2 experiments, *significant difference ( 𝑃 < 0 . 0 5 ) aerosol group versus control group, ** aerosol group versus oral/aerosol group, # oral/aerosol group versus oral group, and ## control group versus aerosol, oral and oral/aerosol groups.