Table of Contents
ISRN Cardiology
Volume 2011, Article ID 831062, 6 pages
Research Article

Modification of a Volume-Overload Heart Failure Model to Track Myocardial Remodeling and Device-Related Reverse Remodeling

1Cardiovascular Surgical Research Laboratories, Texas Heart Institute, Houston, TX 77225, USA
2Texas A&M Institute for Preclinical Studies, 800 Raymond Stotzer Pkwy, Ste 2060, College Station, TX 77843-4478, USA
3Department of Pathology, University of Texas Health Science Center, Houston, TX 77030-1501, USA
4Department of Pediatric Cardiology, Baylor College of Medicine, Houston, TX 77030, USA

Received 4 March 2011; Accepted 2 May 2011

Academic Editor: M. Guazzi

Copyright © 2011 Egemen Tuzun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To provide an ovine model of ventricular remodeling and reverse remodeling by creating congestive heart failure (CHF) and then treating it by implanting a left ventricular assist device (LVAD). Methods. We induced volume-overload heart failure in 2 sheep; 20 weeks later, we implanted an LVAD and assessed recovery 11 weeks thereafter. We examined changes in histologic and hemodynamic data and levels of cellular markers of CHF. Results. After CHF induction, we found increases in LV end-diastolic pressure, LV systolic and diastolic dimensions, wall thickness, left atrial diameter, and atrial natriuretic protein (ANP) and endothelin-1 (ET-1) levels; β-adrenergic receptor (BAR) and dystrophin expression decreased markedly. Biopsies confirmed LV remodeling. After LVAD support, LV systolic and diastolic dimensions, wall thickness, and mass, and ANP and ET-1 levels decreased. Histopathologic and hemodynamic markers improved, and BAR and dystrophin expression normalized. Conclusions. We describe a successful sheep model for ventricular and reverse remodeling.