Table of Contents
ISRN Pharmacology
Volume 2011, Article ID 847980, 8 pages
Research Article

Studies on Wound Healing Activity of Heliotropium indicum Linn. Leaves on Rats

1Institute of Pharmacy and Technology, Salipur, Cuttack District, Odisha 754202, India
2Royal College of Pharmacy and Health Sciences, Andha Pasara Road, Berhampur, Odisha 760002, India

Received 15 January 2011; Accepted 24 February 2011

Academic Editor: F. J. Miranda

Copyright © 2011 G. K. Dash and P. N. Murthy. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The petroleum ether, chloroform, methanol, and aqueous extracts of Heliotropium indicum Linn. (Family: Boraginaceae) were separately evaluated for their wound healing activity in rats using excision (normal and infected), incision, and dead space wound models. The effects of test samples on the rate of wound healing were assessed by the rate of wound closure, period of epithelialisation, wound breaking strength, weights of the granulation tissue, determination of hydroxyproline, super oxide dismutase (SOD), catalase, and histopathology of the granulation tissues. Nitrofurazone (0.2% w/w) in simple ointment I. P. was used as reference standard for the activity comparison. The results revealed significant promotion of wound healing with both methanol and aqueous extracts with more promising activity with the methanol extract compared to other extracts under study. In the wound infection model (with S. aureus and P. aeruginosa), the methanol extract showed significant healing activity similar to the reference standard nitrofurazone. Significant increase in the granulation tissue weight, increased hydroxyproline content, and increased activity of SOD and catalase level with the animals treated with methanol extract in dead space wound model further augmented the wound healing potential of H. indicum. The present work substantiates its validity of the folklore use.