Table of Contents
ISRN Dentistry
Volume 2011, Article ID 870364, 6 pages
http://dx.doi.org/10.5402/2011/870364
Research Article

Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model

1Division of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan
2Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan
3Division of Periodontology, Department of Cariology and Periodontology, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan
4Marketing Research Section, R&D Department, NISSIN DENTAL PRODUCTS INC., 22-1 miyabayashi, asahi, Kameoka, Kyoto 621-0001, Japan
5Department of Conservative Dentistry, Kaohsiung Medical University Hospital and College of Dental Medicine, Kaohsiung Medical University, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan
6Division of Comprehensive Dentistry, Department of Clinical Communication and Practice, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan

Received 13 May 2011; Accepted 19 June 2011

Academic Editor: A. Carrassi

Copyright © 2011 Masato Nagayoshi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Photodynamic therapy has been expanded for use in endodontic treatment. The aim of this study was to investigate the antimicrobial effects of diode laser irradiation on endodontic pathogens in periapical lesions using an in vitro apical lesion model. Study Design. Enterococcus faecalis in 0.5% semisolid agar with a photosensitizer was injected into apical lesion area of in vitro apical lesion model. The direct effects of irradiation with a diode laser as well as heat produced by irradiation on the viability of microorganisms in the lesions were analyzed. Results. The viability of E. faecalis was significantly reduced by the combination of a photosensitizer and laser irradiation. The temperature caused by irradiation rose, however, there were no cytotoxic effects of heat on the viability of E. faecalis. Conclusion. Our results suggest that utilization of a diode laser in combination with a photosensitizer may be useful for clinical treatment of periapical lesions.