Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model

Figure 3

Effects of heat produced by laser irradiation. (A) Change of inner temperature in apical lesion area of in vitro model following laser irradiation. (B) Antimicrobial effects of heat on E. faecalis. The microorganisms were exposed to diode laser irradiation in the presence of a photosensitizer for 60 seconds [5 W-60 s/PS(+)] or exposed to heat at 65°C for 60 seconds (65°C-60 s). (C). Photograph of temperature change monitoring using a thermotracer, which was used to monitor 4 points (a, b, c, d) of the in vitro model. (D–F). Temperature change without photosensitizer. (G–I). Temperature change with photosensitizer. (J) Temperature change in periapical lesion area of the model during irradiation (closed diamond, with photosensitizer; closed square, without photosensitizer). The rise in temperature was calculated as the difference in temperature between points a and b. Data are expressed as the mean ± standard deviation of triplicate determinations. The experiment was performed three times, with similar results obtained in each.