Research Article

Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins

Figure 6

Effect of daidzein (500 μg/g bwt) on level of apoptosis through Hoechst (33258) staining. (a) Pictomicrographs showing apoptotic nuclei in prepubertal female Sprague Dawley rats at PND21 and PND50. (b) mean percent change ± SEM of apoptotic nuclei count, in PND21 as well as PND50 mammary glands. Each bar represents the average ± SEM from 6 animals. * 𝑃 < 0 . 0 5 , ** 𝑃 < 0 . 0 1 , *** 𝑃 < 0 . 0 0 1 , compared to the respective value in control group.
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896826.fig.006b
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