Novel Strategy with Gemcitabine for Advanced Pancreatic Cancer
Figure 2
(a) Drug resistance of pancreatic cancer cells to 5-FU+GEM. Cultured cells ( cells/well) were exposed to graded concentrations of gemcitabine (0, 10-2–104 nM) together with several 5-FU concentrations (0, 0.1, 1.0 μM) for 72 h. Drug resistance was expressed as the concentration of drug that inhibited colony formation by 50% (IC50). The control for each cell line (5-FU, 0 μM) showed statistical significance with each of the same cell lines (*) (). (b) Effect of TS expression on GEM resistance. Knockout of TS expression significantly decreased resistance to GEM in pancreatic cancer cells as evidenced by the detected change in IC50s from nM to nM. Results are expressed as mean ± SD. Bar, SD; TS, thymidylate synthase.
