Table of Contents
ISRN Allergy
Volume 2011, Article ID 950104, 12 pages
Research Article

The Characterization of the Repertoire of Wheat Antigens and Peptides Involved in the Humoral Immune Responses in Patients with Gluten Sensitivity and Crohn's Disease

Immunosciences Laboratory, Inc., 822 S. Robertson Boulevard, Suite 312, Los Angeles, CA 90035, USA

Received 20 July 2011; Accepted 21 August 2011

Academic Editors: B. M. Stadler and P. E. Taylor

Copyright © 2011 Aristo Vojdani. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Intestinal T cells from gluten sensitivity/celiac disease patients respond to a heterogeneous array of peptides. Our study extended this heterogeneity to humoral immune response to various wheat proteins and peptides in patients with gluten sensitivity or Crohn’s disease. IgG and IgA antibodies in sera from those patients and healthy control subjects were measured against an array of wheat antigens and peptides. In gluten-sensitive patients, IgG reacted most against transglutaminase, prodynorphin, wheat extract, and ๐›ผ -, ๐›พ -, and ๐œ” -gliadin; IgA reacted most against wheat then transglutaminase, glutenin, and other peptides. In the sera of Crohn’s disease patients, IgG reacted most against wheat and wheat germ agglutinin then transglutaminase, prodynorphin, ๐›ผ -, and ๐›พ -gliadin; IgA reacted foremost against prodynorphin then transglutaminase and ๐›ผ -gliadin. These results showed a substantial heterogeneity in the magnitude of IgG and IgA response against various wheat antigens and peptides. Measurements of IgG and IgA antibodies against such an array of wheat peptides and antigens can enhance the sensitivity and specificity of serological assays for gluten sensitivity and celiac disease and may also detect silent celiac disease or its overlap with inflammatory bowel disease.