Table of Contents
ISRN Microbiology
Volume 2011 (2011), Article ID 951694, 9 pages
Research Article

Optimization of Chromium Removal by the Indigenous Bacterium Bacillus spp. REP02 Using the Response Surface Methodology

1Division of Food Microbiology and Biotechnology, Department of Food Science and Technology, Pondicherry University, Pondicherry 605 014, India
2Forest Pathology Laboratory, Forest Protection Division, Institute of Forest Genetics and Tree Breeding, Coimbatore 641 002, Tamil Nadu, India
3Karpagam University, Coimbatore 641 021, Tamil Nadu, India
4Department of Microbiology, Faculty of Sciences, Usmanu Danfodiyo University Sokoto, Sokoto, Nigeria

Received 5 July 2011; Accepted 17 August 2011

Academic Editors: D. Liu and D. A. Saffarini

Copyright ยฉ 2011 C. K. Venil et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An indigenous bacterium, Bacillus REP02, was isolated from locally sourced chromium electroplating industrial effluents. Response surface methodology was employed to optimize the five critical medium parameters responsible for higher % Cr2+ removal by the bacterium Bacillus REP02. A three-level Box-Behnken factorial design was used to optimize K2HPO4, yeast extract, MgSO4, NH4NO3, and dextrose for Cr2+ removal. A coefficient of determination ( ๐‘… 2 ) value (0.93), model ๐น -value (3.92) and its low ๐‘ƒ -value ( ๐น < 0 . 0 0 0 8 ) along with lower value of coefficient of variation (5.39) indicated the fitness of response surface quadratic model during the present study. At optimum parameters of K2HPO4 (0.6 g L−1), yeast extract (5.5 g L−1), MgSO4 (0.04 g L−1), NH4NO3 (0.20 g L−1), and dextrose (12.50 g L−1), the model predicted 98.86% Cr2+ removal, and experimentally, 99.08% Cr2+ removal was found.