Altered or Impaired Immune Response to Hepatitis B Vaccine in WNIN/GR-Ob Rat: An Obese Rat Model with Impaired Glucose Tolerance

Table 2

Mitogen stimulated IL2 cytokine production by splenocytes and LPS-stimulated TNF-α and NO production by peritoneal macrophages to Hepatitis B vaccine in 3-month-old WNIN/GR-Ob lean and obese rats.

Immune parameters	WNIN/GR-Ob lean unvaccinated (𝑛=6)	WNIN/GR-Ob obese unvaccinated (𝑛=6)	WNIN/GR-Ob lean vaccinated (𝑛=8)	WNIN/GR-Ob obese vaccinated (𝑛=8)
Con A stimulated IL2 production (ng/mL)	1505 ± 446	1087 ± 149	1423 ± 323	940 ± 290
LPS stimulated TNF-α release (ng/mL)	1642 ± 748^a,b	430 ± 17^a,b	1974 ± 449^*a	384 ± 28^b
LPS stimulated NO production (ng/mL)	1.96 ± 0.35^*a	4.4 ± 0.35^b	4.7 ± 0.66^b	4.25 ± 1.34^a,b

Values are in mean ± SE; *𝑃<0.05 (significant difference between unvaccinated and vaccinated groups of lean and obese rats). The means bearing similar superscripts in each row do not differ significantly.