Tissue Distribution and Associated Toxicological Effects of Decabrominated Diphenyl Ether in Subchronically Exposed Male Rats

Table 1

Sequences of primers used for real-time RT-PCR amplification.

Target gene	GeneBank accession no.^a	5′-3′ Primer sequences^b	Product length (bp)	Tm (°C)
β-actin	NM_031144	FW: TCGTGCGTGACATTAAAGAG	134	56
		RW: ATTGCCGATAGTGATGACCT
CYP1A2	NM_000761	FW: GTGGAATCGGTGGCTAAT	105	54
		RW: CACAAAGTCCTTGCTGCTC
CYP2B1	NM_001134844	FW: GCCTCCTCAATTCCTTCA	99	53
		RW: TGTCTGTCCCACATAGCAT
CYP2B2	XM_001062335	FW: AGGAGAAGTCGAACCACCAC	82	56
		RW: GAGCAGGAAACCATAGCG
CYP2C6	XM_001066767	FW: TGTAGAGTTTCAGGGATGG	94	50
		RW: AGCAGTGAGATTGGGAAG
CYP3A2	NM_153312	FW: GTCTCATAAAGCCCTGTC	81	47
		RW: CTGCTGGTGGTTTCATAG

^aGeneBank accession number used to design the primers.
^bFW: forward primer; RW: reverse primer.