Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 101092, 5 pages
Research Article

Determination of Picogram Levels of Roxithromycin in Pharmaceutical, Human Serum, and Urine by Flow-Injection Chemiluminescence

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710069, China

Received 20 August 2011; Accepted 25 September 2011

Academic Editors: V. Maier and A. Taga

Copyright © 2012 Jiangman Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A sensitive chemiluminescence (CL) method, based on the inhibitory effect of roxithromycin (ROX) on the CL reaction between luminol and dissolved oxygen in a flow-injection system, was first proposed for the determination of ROX at picogram levels. The decrement of CL intensity was linearly proportional to the logarithm of ROX concentrations ranging from 0.1 to 100 pg mL−1, giving the limit of detection (LOD) of 0.03 pg mL−1 (3σ). At a flow rate of 2.0 mL min−1, a complete analytical procedure including sampling and washing could be performed within 0.5 min, with relative standard deviations (RSDs) of less than 5.0% ( 𝑛 = 5 ). The proposed procedure was applied successfully to the determination of ROX in pharmaceutical, human serum, and urine with the recoveries ranging from 90.0 to 110.0%.