Table of Contents
ISRN Toxicology
Volume 2012, Article ID 121926, 4 pages
Research Article

Determination of Aflatoxin in Milk by ELISA Technique in Mashad (Northeast of Iran)

1Department of Food Science & Technology, Islamic Azad University, Quchan Branch, Quchan, Iran
2Quality Control Laboratory of Food & Drug Administration, Mashhad University of Medical Sciences, Khorasan, Mashhad 77558 3918, Iran

Received 2 July 2012; Accepted 7 August 2012

Academic Editors: P. J. Boogaard, R. A. Manderville, and B. Zhou

Copyright © 2012 Ali Mohamadi Sani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to detect the amount of aflatoxin M1 (AFM1) in pasteurized milk samples in Mashad in northeast of Iran. For this purpose, 42 milk samples were collected from retail stores during fall 2011 and analyzed for AFM1 by enzyme-linked immunosorbent assay (ELISA) technique. All the analyses were done twice. Results showed presence of AFM1 in 97.6% of the examined milk samples by average concentration of 23 ± 16 ppt and contamination level ranging between 6 and 71 ppt. The concentration of AFM1 in all the samples was lower than the Iranian national standard and Food and Drug Administration limits (500 ppt), and, only in 3 (1.6%) samples, AFM1 concentration was more than the maximum tolerance limit (50 ppt) accepted by European Union and Codex Alimentarius Commission. According to our findings and previous studies, AFM1 contamination of milk is not a concern in this region, and the regional standard of AFM1 contamination in milk might be changed to lower than 100 ppt.