Table of Contents
ISRN Agronomy
Volume 2012, Article ID 121983, 8 pages
Research Article

Physiological Responses of Wild and Cultivated Barley to the Interactive Effect of Salinity and Iron Deficiency

Laboratoire d’Adaptation des Plantes aux Stress Abiotiques (LAPSA), Centre de Biotechnologie de Borj-Cédria, P.O. Box 901, 2050 Hammam-lif, Tunisia

Received 7 April 2012; Accepted 10 May 2012

Academic Editors: K. Okuno and L. Zeng

Copyright © 2012 Sabeh Yousfi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Literature on the separate effects of salinity and inadequate Fe supply on plant growth and nutrient uptake, concentration, and distribution is abundant but little is known about the interactive effects of these two abiotic constraints. Here, we investigated the interactive effect of iron availability and salinity on physiological responses of cultivated and wild barley (Hordeum vulgare and H. maritimum resp.). Seedlings of both species were grown for 9 days, under complete nutrient solution with or without iron supply. Then, NaCl treatment was applied at different concentrations (0, 100, 200, and 300 mM) for 60 hours. After salt exposure, shoot water content of H. vulgare was significantly reduced as compared to H. maritimum. Furthermore, Na+ accumulation in shoots increased parallel to increasing NaCl concentration in the medium. However, the increase was significantly higher in H. vulgare than in H. maritimum. These responses were associated with lower Fe absorption efficiency photosynthetic parameters in both species. The reduction was significantly higher in cultivated than in wild barley. Moreover, phytosiderophore exudation was enhanced in both species by direct (iron free medium) or indirect iron limitation (salt-induced iron limitation). Such a stimulation of phytosiderophore release was genotype and salt level dependant.