(a) Schematic representation of transfection and cultivation procedure
(b) mRNA after in vitro transcription
Figure 1: Generation of iPS cells using mRNA. (a) Schematic representation of the method: an initial nucleofection is followed by three lipofections at 72 h intervals and a “resting” period of 3 weeks, after which colony formation is observed. Fluorescence protein (RFP) demonstrates transfection efficacy in the early stages, colony formation at later stages is shown. (b) Examples of the mRNA quality measurement with an Agilent bioanalyzer. The single peaks indicate that only one type of RNA is produced.