International Scholarly Research Notices / 2012 / Article / Fig 4

Research Article

Reprogramming of Human Huntington Fibroblasts Using mRNA

Figure 4

In vitro differentiation of iPS cells. (a) Spontaneous differentiation into embryoid bodies (EBs) was followed for several days and EBs stained positive for various differentiation markers. (b) mRNA-iPS cells derived from human fibroblasts (female, 21 yrs) were differentiated towards hepatocytes, neurons, and chondrocytes (see Experimental Procedures). Cells expressed lineage-typical markers, (hepatocytes: AFP and cytokeratin 18; neurons: GFAP and tubulin III; chondrocytes: collagen II and aggrecan) and showed morphological changes. All cells were also stained with DAPI, and images were merged with lineage markers.
(a) Adult mRNA-iPS cells: Spontaneous differentiation
(b) Directed differentiation

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.