Central Role of Ubiquitination in Genome Maintenance: DNA Replication and Damage Repair

Figure 1

Fanconi’s anemia pathway. DNA ICLs are recognized by a recognition complex consisting of FANCM, protein FAAP-24, and histone fold proteins MHF1 and MHF2. This complex then loads the FA core complex comprising of FANC (A, B, C, E, F, G, L, and FAAP-10) subunits and having the E3 ligase activity. FANCL, the catalytic subunit of the complex, interacts with the E2 conjugating enzyme. FANCL binds FANCD2 and FANCI, monoubiquitinates them, and finally recruits them to chromatin. Monoubiquitinated FANCD2 is finally targeted to the repair foci along with BRCA2, FANCJ, FANCN, and other proteins to carry out the repair function.