Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells: An Ultrastructural Analysis

Figure 2

Transmission electron micrographs of human granulosa-lutein cells (GLCs) collected only with PBS cultured for 144 hs in TCM-199 medium. (a) Disrupted GLCs cytoplasm containing innumerous vacuoles and several bacteria. (b) Details of bacteria cytoplasm (*) and wall (arrows). (c) GLCs debris showing enclosed bacteria. (d) Detail of undefined and electron dense GLC nuclei, digestive vacuole (dotted arrow), and bacteria wall (arrow). N: nuclei, V: vacuoles. TEM: (a) X 27,000. (b) X 5,000. (c) X 6,700. (d) X 27,000.