Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells: An Ultrastructural Analysis

Figure 3

Effect of bacterial contamination on human granulosa-lutein cells (GLCs) steroid secretion. Concentrations of media estradiol (a) and progesterone (b) after 144 hours of culture. GLCs collected with PBS containing antibiotic/antimycotic (noninfected group) or collected only with PBS (infected group). Data shown as mean ± SEM; ***𝑃<0.0001; **𝑃<0.005 versus noninfected group.

(a)

(b)