An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis
Figure 1
TGF induced EMT in A549 cells. Epithelial (a) and mesenchymal (b) gene expression in A549 cells stimulated with TGF (0.01, 0.1, 1, 10, and 100 ng/mL) vehicle control or media alone for 24 hours. Gene expression levels were determined using QuantiGene 2.0 branched DNA technology. Data was analysed relative to the expression of the endogenous control (PPIB) and normalised to unstimulated cells. Data shown represents mean ± SEM of three independent experiments (). A549 cell morphology changes following TGF (0.01, 0.1, 1, 10, and 100 ng/mL) or media alone treatment for 72 hours, live cells were visualised using phase contrast microscopy, and representative images are shown (c). Immunocytochemistry of the mesenchymal markers; fibronectin-EDA, vimentin and collagen I (red), and nuclei (blue) in A549 cells treated with TGF (1 and 100 ng/mL) or media alone for 72 hours. Cells were visualised using an ArrayScan (Cellomics), and representative images are shown (d). *, ** statistically significant versus vehicle control, using a one-way ANOVA with Dunnett’s posttest.
