An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis
TGF induced EMT in NHBE cells. Epithelial (a) and mesenchymal (b) gene expression in NHBE cells stimulated with TGF (1 and 10 ng/mL) or media alone for 24 hours. Gene expression levels were determined using QuantiGene 2.0 branched DNA technology. Data was analysed relative to the expression of the endogenous control (PPIB) and normalised to unstimulated cells. Data shown represents mean ± SEM of at least three independent experiments (). (c) Effects of TGF-induced EMT on E-cadherin and fibronectin-1 protein levels assessed by western blot and quantified using densitometry. Data shown represents mean ± SEM of at least three independent experiments (). (d) Representative western blot evaluation of NHBE cells treated with 10 ng/mL of TGF (+) or media alone (−) was performed after 48 hours to determine levels of E-cadherin and fibronectin-1. -tubulin loading control was used. Data is shown from one experiment.