An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis
Evidence of increased collagen deposition and changes in epithelial, mesenchymal, and apoptotic gene expression following lung-specific TGF transgene overexpression. TGF was overexpressed by introducing doxycycline (0.5 mg/mL) into the animals water; wild-type controls also received doxycycline for 3, 7, 10, and 21 days. Total soluble collagen was measured in homogenates from the left lobe of the lung using the Sircol assay (a). RNA was isolated from whole lung and qRT-PCR was performed to identify EMT (b) and proapoptotic (c) regulated genes. Fold change in gene expression was calculated by comparing TGF mice with wild-type mice at matching time points. Data represent mean ± SEM of at least mice at each time point. * using Student’s t-test.