162802.fig.002a
(a)
162802.fig.002b
(b)
Figure 2: (a) At 24 hours, high glucose resulted in significant mitochondrial membrane hyperpolarization. Subsequently, endothelial mitochondria were depolarized (after 48 hours). Treatment with C-peptide resulted in restoration of Δ Ψ m at both 24 and 48 hours of exposure to a high glucose stress. *, significantly greater than low-glucose and high-glucose + C-peptide ( 𝑃 < 0 . 0 0 9 ); #, significantly less than low-glucose and high-glucose + C-peptide ( 𝑃 < 0 . 0 0 2 ). Data represent means ± SEM and 𝑛 = 6 experiments. (b) Representative fluorescence micrographs of renal MECs treated with JC-1.