Figure 5: Cell supernatants obtained after 24 hr treatments were subjected to western blotting analysis for detection of phosphorylated ERK isoforms (p44 kDa/pERK1 and p42 kDa/pERK2) and Phosphorylated p38 MAPK isoforms. (a) Phosphorylated MAPK band intensities were normalized to total MAPK levels. *significantly greater than all other groups ( 𝑃 < 0 . 0 0 0 5 ) . Data are expressed as percent change relative to control low glucose treatment. Data represent mean ± SEM, 𝑛 = 6 . (b) Representative western blot images.